Facile and rapid manipulation of DNA surface density on gold nanoparticles using mononucleotide-mediated conjugation.

نویسندگان

  • Wenting Zhao
  • I-Ming Hsing
چکیده

We report here a method to manipulate the amount of thiolated DNA attached per gold nanoparticle from a few to tens of strands in less than an hour, which involves the tuning of the respective amount of salt and time of addition of thiolated oligo(ethylene glycol) during mononucleotide-mediated conjugation process.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Biofunctionalization of Gold Nanorods: A Comparative Study on Conjugation Methods for Fabrication of Nanobiosensors

Gold Nanorods have promised variety of applications in biomedicine and biosensing. As a fruitful candidate for early detection and imaging, these plasmonic nanoparticles have been utilized for diagnostic applications of interest. However, prior to design and fabricate SPR-based nanobiosensors, the type and nature of conjugation with biomolecules would be of utmost importance. Herein, four strat...

متن کامل

Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers

BACKGROUND Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context, in situ conjugation of nanoparticles with biomolecules via laser ablation in an aqueous media is a highly promising one-step method for the production of functional nanoparticles resulting in highly efficient conjugation. Increased yields are required, parti...

متن کامل

Reversible luminescence switching of NaYF4:Yb,Er nanoparticles with controlled assembly of gold nanoparticles.

Upon the tunable surface plasmon band of the pH-induced reversible assembly of gold nanoparticles mediated by cysteine, the manipulation of green and red upconversion emission, and the switching of the red emission of the NaYF(4):Yb,Er nanoparticles have been achieved in a facile and reproducible way.

متن کامل

A colorimetric aptasensor for selective detection of oxytetracycline in milk, using gold nanoparticles and oxytetracline-short aptamer

Objective (s): In light of misuse of antibiotics in animal husbandry and their side effects on human health, there is an argent need to develop simple and rapid methods for determining the quantification of antibiotics in biological systems. Materials and Methods: In this work a facile and ultrasensitive colorimetric aptasensor was reported for detection of oxytetracycline (OTC) in water and mi...

متن کامل

Gradual Growth of Gold Nanoseeds on Silica for Silica@Gold Core-Shell Nanoparticles and Investigation of Optical Properties

Metal nanoshells consists of a dielectric core surrounded by a thin noble metal shell, possess unique optical properties that render nanoshells attractive for use in different technologies. This paper reports a facile method for growth of small gold nanoparticles on the functionalized surface of larger silica nanoparticles. Mono-dispersed silica particles and gold nanoparticles were prepared by...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 46 8  شماره 

صفحات  -

تاریخ انتشار 2010